COLL 398 |
| The insertion of transmembrane protein channels into supported lipid bilayers for ion-channel measurements has been an integral tool for investigating the biophysical properties of protein channels, as well as for quantifying the interactions of protein channels with receptor molecules. Recently, application of protein ion channels supported in lipid bilayers has been proposed for designing biosensors and the sequencing of DNA. In this presentation, protein ion-channel recordings using a glass nanopore membrane (GNP, ~200 nm radius orifice) as the support structure for lipid bilayer membranes are described. The GNP membrane/bilayer structures, which exhibit ohmic seal resistances of ~70 gigaohm and electrical breakdown voltages of ~0.8 V, are exceptionally stable to mechanical disturbances, and have lifetimes of at several weeks. These favorable characteristics result from the very small area of bilayer that is suspended across the GNP membrane orifice. Several applications of the GNP ion channel recording device in single-molecule and single-particle analyses will be presented. |
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Electrochemistry-Enabled Nano S&T
2:00 PM-5:15 PM, Wednesday, April 9, 2008 Morial Convention Center -- Rm. 229, Oral
Division of Colloid & Surface Chemistry |