Oxygen dissociation from red blood cells in a perfluorocarbon solution

CHED 1312

Adam J. Hayek, Adam.Hayek@loras.edu, Department of Biochemistry, Loras College, 1450 Alta Vista Street, Dubuque, IA 52001, David J. Oostendorp, david.oostendorp@loras.edu, Department of Chemistry, Loras College, 1450 Alta Vista, Dubuque, IA 52001-0178, and David Speckhard, Department of Chemistry and Biochemsitry, Loras College, 1450 Alta Vista, Dubuque, IA 52001.
Using visible and near infrared spectroscopy to investigate the effects of a perfluorocarbon solution on the dissociation of oxygen from purified bovine red blood cells. Primarily, the oxygen dissociation curve of bovine red blood cells is found in a phosphate buffer solution through tonometry and then compared to known values. Next, the phosphate buffer solution is replaced by an isotonic and pH buffered perfluorocarbon solution. This perfluorocarbon solution will closely represents a new blood replacement therapy. The two oxygen dissociation curves are then compared and contrasted.
 

Undergraduate Research Poster Session: Medicinal Chemistry
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Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008