Serum and saliva CEA determinations via fluorescent nanoparticles

ANYL 149

Jesse V. Jokerst, jokerst@mail.utexas.edu, Amanda Pollard, and John T. McDevitt, mcdevitt@mail.utexas.edu. Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712
Carcinoembryonic antigen (CEA) is an oncofetal glycoprotein normally present in utero, with elevated adult levels correlated to a number of cancers. Whole saliva and serum samples were collected from control and cervical cancer volunteers. Fluorescence signal arose from quantum dot (QD) labeled detecting antibody after antigen immobilization on agarose beads via sandwich-type assay. Limit of detection for CEA was reduced to 0.02 ng/mL in the sensor, nearly two orders of magnitude lower than that of ELISA systems. The QD fluorophores give nearly 30x amplification of signal versus Alexa 488. Samples from diseased individuals showed consistently elevated CEA levels compared to their healthy counterparts in both saliva and serum. Strong correlation was observed between the two analytical techniques. This new platform in tandem with next generation fluorophores promises to be a robust tool for analysis of complex fluids over range of concentrations.
 

Nanomaterials in Analytical Chemistry
1:25 PM-5:05 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral

Division of Analytical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008