Comparative study of the kinetics of MS2 bacteriophage and Coxsackievirus B5 inactivation with monochloramine

ENVR 267

Benjamin J. Finnegan, bfinnega@uiuc.edu1, Joanna L. Shisler, jshisler@life.uiuc.edu2, and Benito J. Mariņas, marinas@uiuc.edu1. (1) Department of Civil and Environmental Engineering, Center of Advanced Materials for the Purification of Water with Systems, University of Illinois at Urbana-Champaign, Urbana, IL 61801, (2) Department of Microbiology, Center of Advanced Materials for the Purification of Water with Systems, University of Illinois at Urbana-Champaign, C222 CLSL, 601 S Goodwin Ave, Urbana, IL 61801
Many U.S. drinking water utilities currently utilize combined chlorine (monochloramine) for primary and/or secondary disinfection. The EPA's Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESTR) should force more utilities to switch to using combined chlorine to minimize the formation of toxic disinfection byproducts associated with free chlorine, currently the disinfectant most commonly used in the U.S. The effectiveness of combined chlorine disinfection on viruses has not been well characterized and water utilities are not able to fully elucidate the effect of their treatment processes on viruses. In this study, the effect of monochloramine disinfection on two ssRNA viruses, MS2 bacteriophage and Coxsackievirus B5, is investigated. The goal of this study is to determine the effect of disinfectant concentration, pH and temperature on viral inactivation. Comparing the results will allow assessing the adequacy of MS2 as a surrogate for enteroviruses such as Coxsackievirus.