CHED 831 |
| Amphitrite ornata Dehaloperoxidase (DHP) is a heme-containing enzyme with the capability of oxidatively dehalogenating halogenated organic compounds, an environmentally significant ability due to the number of halogenated organics that are pollutants. As the mechanism of action for DHP is still under investigation, this senior research project focuses on the investigation of the chemical reactivity of this enzyme by developing a biological model system using a well-characterized heme enzyme, Cytochrome c Peroxidase (CCP). Here, we utilize site-directed mutagenesis to introduce a R48A/W51A mutation into the distal heme pocket of CCP to create a more spacious active site potentially capable of binding a halogenated organic compound. Following the purification of the R48A/W51A CCP mutant, we will analyze its binding and catalytic capabilities. Results from this study will offer further insight into dehaloperoxidase chemistry and possible correlations to the mechanism of action of DHP. |
|
Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |