Synthesis and mechanical property characterization of novel ionenes for biomedical applications

POLY 278

Sharlene R. Williams1, Erika M. Borgerding1, John M. Layman2, and Timothy E. Long, telong@vt.edu1. (1) Department of Chemistry, Virginia Tech, Blacksburg, VA 24061, (2) Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061
Ammonium ionenes were prepared via the Menshutkin reaction from 1,12-dibromododecane or 1,6-dibromododecane and 1,12-bis(N,N-dimethylamino)dodecane. The absolute molecular weights were determined for the first time using an on-line multi-angle laser light scattering (MALLS) in aqueous size exclusion chromatography (SEC). Structure-property relationships between molecular weight and mechanical properties were established for a series of 12,12-ammonium ionenes using both tensile testing and dynamic mechanical analysis (DMA). Tensile analysis of the higher molecular weight ionenes revealed an average stress at yield of 22 MPa and elongations ranging from 233-441%. To determine if the ionenes were suitable for biological applications, a thorough analysis was conducted on SY5Y and COS-7 cell lines to determine the cytotoxicity of the ionenes at various concentrations. Specifically, Promega CellTiter 96® non-radioactive cell proliferation and Cell Titer-Glo® luminescent cell viability assays were compared. Concentrations of ionenes ranged from 1-800 µg/mL, and significant cell viability was observed at concentrations less than 25 µg/mL.
 

Functional Nanomaterials from New Polymer Synthetic Methodologies
2:00 PM-5:50 PM, Monday, April 7, 2008 Hilton New Orleans Riverside -- Grand Salon 7/10, Oral

Division of Polymer Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008