Cytotoxicity and related inflammatory response for some manufactured metal oxide and carbon nanoparticulate material aggregates

ENVR 56

K. M. Garza, kgarza@utep.edu, Department of Biological Sciences, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, L. E. Murr, lemurr@utep.edu, Department of Metallurgical and Materials Engineering, The University of Texas at El Paso, 500 W. University Ave., Room M-201, El Paso, TX 79968-0520, and K. F. Soto, karlafs@gmail.com, Lockheed Martin Aeronautics Company, 1000 Lockheed Martin Blvd., Fort Worth, TX 76108.
We have utilized a range of manufactured or commercial nanoparticulate materials, including carbon nano-PM, to investigate and compare their cytotoxic response in vitro with an immortalized human epithelial (lung model) cell line (A549). These have included Al2O3, TiO2, Fe2O3 and ZrO2, black carbon (BC) and two types of MWCNT-aggregate PM (MWCNT-R and MWCNT-N). Two-day and two-week in vitro cultures of A549 showed cell death (or decreased cell viability) for all nanoparticulate materials, except the TiO2. Cytokine release (IL-6) was detected for the Fe2O3, BC and the MWCNT PM. Reactive oxygen species (ROS) production was also detected for Fe2O3, ZrO2, BC and the MWCNT aggregate PM. TEM and FESEM examination of these nanomaterials illustrate a wide range in PM morphologies, crystallinities and other physico-chemical phenomena. Taken together, these results illustrate proinflammatory and related respiratory health issues in relation to a range of manufactured nanoparticulates.
 

Environmental Behavior and Fate of Manufactured Nanomaterials
8:30 AM-12:10 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 235, Oral

Division of Environmental Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008