PHYS 39 |
| We have developed approaches to improve the intrinsic properties of fluorophores nearby metal particles. The rate of excitation and emission thus increased due to local electric fields of metal particles induced by incident light. Finite-difference time-domain (FDTD) simulations were employed to calculate the near-field distribution around the excited fluorophores in the absence and presence of metal particles. We believe that the metal-associated effects come from the fluorophore-metal complexes instead of individually from either the fluorophores or metal particles. The metal-associated fluorescence has been applied experimentally on metal-enhanced fluorescence (MEF) or metal-enhanced FRET. The fluorescence was also noted to be coupled through thin metal film with special angle so called surface plasmon-coupled emission (SPCE) due to excitation of metal plasmon by nearby fluorophore that radiates into the glass substrate at the surface plasmon resonances (SPR) angle. We are using metal-associated fluorescence to develop highly sensitive biological and clinical assays. |
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Spectroscopy, Chemistry, and Imaging through Nanophotonics
8:00 AM-12:05 PM, Sunday, April 6, 2008 Morial Convention Center -- Rm. 340/341, Oral
Division of Physical Chemistry |