Enantioseparation of profen drugs by capillary electrophoresis: Using vancomycin and cyclodextrin as chiral selectors

CHED 252

Rebecca A. Hunter, rah15@hood.edu and Kevin H. Bennett. Department of Chemistry and Physics, Hood College, 401 Rosemont Avenue, Frederick, MD 21701
Capillary electrophoresis (CE) was used to achieve enantiomeric separation of the 2-arylpropionic acid nonsteroidal anti-inflammatory drugs (2-APA NSAIDs) naproxen, flurbiprofen, ketoprofen, and ibuprofen. Heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin (TM-β-CD) and the macrocyclic antibiotic vancomycin were used as running buffer chiral selectors (CS). Studies on the effects of buffer pH and CS type and concentration yielded significant differences in enantiomeric resolution under various conditions. It was determined that higher CS concentrations and lower pH buffers lead to greater resolution, along with a significant increase in retention time of the samples. Enantiomeric resolution was achieved for all individually tested racemic NSAIDs for both CS.