CARB 53 |
| DNA:RNA hybrid structures have attracted recent attention due to the key roles they play in a number of biological processes. DNA:RNA hybrids can be found in transcription, reverse transcription and in the priming of DNA prior to transcription. Synthesis and DNA:RNA binding of novel Methidium-Neomycin conjugates will be presented. The conjugates combine two ligands, one known to intercalate duplexes (methidium) and another known to bind in the hybrid duplex major groove (neomycin). The conjugate stabilizes DNA:RNA hybrid duplexes more effectively than neomycin, ethidium or a combination of both. UV, CD and ITC studies show a clear preference for the drug binding to the hybrid duplex over the duplex/single strand structures. Calorimetric measurements show the affinity of the conjugate to be in the nanomolar-picomolar range, surpassing the highest affinity of any aminoglycoside ever known to bind a nucleic acid structure. |
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General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Carbohydrate Chemistry |