ANYL 299 |
| Packed column SFC has come a long way from the time it was introduced in the nineteen seventies. Today's systems use HPLC packed columns that are readily available. Unlike reversed phase HPLC, SFC with packed columns is a normal phase procedure, in which CO2 at its critical temperature and pressure resembles hexane. To solubilize the solutes in the fluidic CO2 a polar modifier is used. pSFC is a fast separation technique that is well suited for clinical applications. In this presentation we will discuss several experimental parameters that influence the pSFC separation. These include effect of modifier type and flow rate, gradient shape, and mixed packing materials on the separation of a series of estrogen metabolites. Compared with HPLC/MS analysis, in terms of speed and sensitivity, pSFC/MS is faster with comparable LOD and LOQ. The role of pSFC in the clinical laboratory will be discussed and commented upon. Funded by NCI Contract N01-CO-12400 |
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Chromatographic Separations in Clinical Chemistry
9:00 AM-11:50 AM, Monday, August 20, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |