BIOL 158 |
| Botulinum neurotoxin type A (BoNT/A), one of the seven neurotoxin prototypes (BoNT/A to BoNT/G), the most toxic substance known to mankind, is produced by Clostridium botulinum type A as a complex with a group of neurotoxin-associated proteins (NAPs) through polycistronic expression of a clustered group of genes. NAPs are known to protect BoNT against adverse environmental conditions and proteolytic digestion. In our lab, we cloned a double mutation recombinant BoNT/A (DRBoNT/A), which is mutated in two amino acids, both in light chain domain of BoNT/A (E224 and E262). Hn-33 is a part of the large complex of BoNT and is critical in toxin protection. In this project, we used yeast two hybrid system to confirm the interaction between DRBoNT/A and Hn-33, suggesting a direct interaction between the BoNT/A and the Hn-33 in the BoNT/A complex. The research was supported in part from a grant from DTRA (W911 NF-06-1-0095). |
|
Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |