BIOHW 3 |
| Human adenovirus Ad-36 causes adiposity in animal models and shows association with human obesity. Humans naturally infected with Ad-36 show greater preadipocyte differentiation. Our objective is to identify the mechanism of Ad-36 induced obesity. Human adipose derived stem cells (hASC) infected with Ad-36 show spontaneous commitment to the adipogenic lineage, differentiation to adipocytes and lipid accumulation. Ad-36 induced lipid accumulation in hASC increases in response to the viral load and the lipogenic response is observed regardless of the donor gender and over an age range of 22-57y. Furthermore, Ad-36 E4 Orf-1 gene is necessary and sufficient for the adipogenic effects of the virus. These results suggest that the ability to induce adipogenesis may contribute to Ad-36 induced adiposity in-vivo. |
|
Genomics of Obesity
8:30 AM-11:45 AM, Monday, August 20, 2007 BCEC -- 203, Oral
Biotechnology of Health and Wellness |