BIOHW 7 |
| The dyslipidemia of obesity is associated with altered plasma triglyceride metabolism and is improved by both total caloric restriction and reduced carbohydrate intake. We studied gene expression in adipose tissue to identify molecular pathways responsive to these dietary changes. Subcutaneous adipose biopsies were obtained from 131 overweight men on four diets varying in carbohydrate content both before and after a 4.5 kg weight loss induced by caloric restriction on each diet. Genome-wide analysis in a subset of subjects showed reduced expression of 30 genes, predominantly involved in energy metabolism and lipogenesis, with both caloric restriction and isoenergetic dietary carbohydrate reduction. Real-time PCR in the entire group confirmed results for four of the most responsive genes (p<0.003), two of which (SCD and DGAT2) were correlated with reductions in plasma triglyceride levels. Thus, transcriptional regulation of these lipogenic genes may be involved in modulating dietary effects on systemic triglyceride metabolism in obesity. |
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Genomics of Obesity
1:30 PM-4:25 PM, Monday, August 20, 2007 BCEC -- 203, Oral
Biotechnology of Health and Wellness |