ANYL 372 |
| Supercritical Fluid Chromatography (SFC) has been successfully implemented as an isolation and purification tool at the pharmaceutical Discovery and Development stages of the pipeline as a result of many notable advantages over HPLC. SFC is normal phase chromatography that replaces the conventional liquid mobile phase with a super critical fluid typically using CO2 and maintaining it over a critical pressure and temperature. The use of CO2 for purification purposes has many advantages. It is inexpensive to purchase and dispose of, reduces fraction evaporation time and costs, is non-toxic, a green chemistry alternative and has lower viscosity and higher diffusivity allowing for higher column efficiencies and better resolution of target peaks. Semi-preparative SFC is an orthogonal approach for the purification of Pfizer's medicinal chemists chiral molecular targets and has become the method of choice in part due to the decreased turn around time and enormous time savings generated at almost every step of the purification process. The Pfizer Structure Elucidation Group has recently implemented SFC into its isolation process of low level achiral drug related target impurities. This serves as a first step in the structure elucidation of low level impurities that are needed to support a drug candidate through the development process. This presentation will touch on the advantages of using SFC compared to HPLC and highlight the successes of using SFC for both Discovery's chiral purification and the Structure Elucidation Groups achiral isolation needs. Case studies will illustrate how SFC has added value within Pfizer. |
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Fast Preparative Chromatography in Pharmaceutical Drug Discovery
1:30 PM-4:50 PM, Tuesday, August 21, 2007 BCEC -- 105, Oral
Division of Analytical Chemistry |