ANYL 371 |
| Supercritical fluid chromatography became a powerful complementary purification method to HPLC for the alternative separation mechanisms it provides to RP-HPLC, in addition to the advantages of faster speed, cost efficiency, and environmental friendliness. These advantages are especially beneficial for a high throughput purification lab. A commercially available prep-SFC system was customized with an open bed fraction collector and in-house developed software, which allowed the collection of a large number of fractions for one injection and data transfer to LIMS software. Aerosol formation was minimized by “dribbler tube” design and excellent recovery was obtained. The compounds that were not resolved in prep-HPLC were well resolved in prep-SFC. Compounds with poor solubility in MeOH/DMSO were successfully purified by prep-SFC with DCM as injection solvent. Based on the success of prep-SFC, various novel stationary phases were also investigated to obtain better resolutions for complicated library purifications. |
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Fast Preparative Chromatography in Pharmaceutical Drug Discovery
1:30 PM-4:50 PM, Tuesday, August 21, 2007 BCEC -- 105, Oral
Division of Analytical Chemistry |