ANYL 302 |
| HPLC with electrochemical detection (HPLC-ECD) has inherent selectivity and sensitivity. Coulometric cells with porous flow-through working electrodes are the most commonly used form of ECD in high volume laboratories. By combining isocratic or gradient HPLC with multiple coulometric sensors, solutes are resolved both chromatographically and by differences in oxidation-reduction behavior. This has allowed the adoption of methods that have higher specificity and require less sample pre-treatment than traditional HPLC-ECD methods. This presentation will focus on the recent expansion of HPLC-ECD usage in clinical laboratories, including analysis of plasma metanephrines, plasma vitamin K, and oxidized and reduced forms of Co-enzyme Q in plasma. Also discussed is the use of an immobilized enzyme reactor in-line with HPLC-ECD for rapid analysis of choline in whole blood or plasma. Other important applications including multivariate profiling of lipid-soluble vitamins and anti-oxidants and individual quantitation of plasma 25-Hydroxyvitamin D2 and D3 will be discussed. |
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Chromatographic Separations in Clinical Chemistry
9:00 AM-11:50 AM, Monday, August 20, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |