UPLC with tandem mass spectrometry for multiresidue determination of sulfonamide antibacterials in milk and tissue

ANYL 39

Michael S. Young, michael_s_young@waters.com, KimVan Tran, and Mark Benvenuti. Waters Corporation, 34 Maple Street, Milford, MA 01757
Sulfonamide antibacterials are commonly used to maintain the health of animals for meat and milk production. Although there are some differences in regulations worldwide, maximum residue levels (MRLs) are typically 10 µg/L for each analyte residue in milk or 100 µg/kg in tissue. In this presentation we will compare precursor ion mass spectrometry with product ion mass spectrometry for multiresidue determination of 10 sulfonamide antibacterials in milk and tissue. The analytes are first isolated from milk or tissue extracts using mixed-mode cation-exchange SPE. After isolation and cleanup, the extracts are analyzed by UPLC-MS-MS in both ion modes. The goal is a simple and rapid method with limits of quantitation (LOQ) well below 10 ng/mL for each sulfonamide.