Smc1, a non-abc protein involved in transport of xenobiotics

BIOHW 12

Sushma Yadav, syadav@uta.edu1, Sharad S Singhal1, Jyotsana Singhal2, Kenneth Drake1, and Sanjay Awasthi1. (1) Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, CPB312, Arlington, TX 76019, (2) University of Texas at Arlington
Ralbp1 is a multispecific membrane efflux protein crucial for efflux of reactive metabolites originating from phase II biotransformation of xenobiotics. Loss of Ralbp1 expression in gene-knockout mice result in a phenotype characterized by sensitivity to xenobiotic as well as radiant stress. Comparison of the gene expression between Ralbp1-/- and Ralbp1+/+ mice was performed to try to ascertain whether the function of RLIP was being replaced by another as yet unknown transporters. Among the most consistent changes in all tissues analyzed were the increase in heat-shock proteins, structural maintenance of chromosome1 (SMC1). SMC1, is implicated in sister chromatid cohesion, DNA recombination and repair, and activation of the cell cycle checkpoints by ionizing radiation, ultraviolet light, and other genotoxic agents. Structural comparison of SMC1 with Ralbp1 indicated the presence of certain structural features found in glutathione-binding proteins, and led to present studies in which we demonstrate homologous functions of SMC1 and Ralbp1.
 

Genomics of Obesity
8:15 AM-12:05 PM, Tuesday, August 21, 2007 BCEC -- 203, Oral

Biotechnology of Health and Wellness

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007