GEOC 34 |
| A simple, fast, sensitive and accurate reversed-phase ion-pair HPLC method for simultaneous determination of nitrite and nitrate in atmospheric liquids and lake waters has been developed. Separations were accomplished in less than 10 min using a reversed-phase C18 column (150 x 2.00 mm i.d., 5 µm particle size) or 5 min utilizing 5.0 x 4.6 mm, 3 µm C18 column with a mobile phase containing 83% 3.0 mM ion-interaction reagent tetrabutylammonium hydroxide (TBA-OH) and 2.0 mM sodium phosphate buffer at pH 3.9 and 17% acetonitrile (flow rate, 0.4 ml/min). UV light absorption responses at 205 nm were linear over a wide concentration range from one hundred µg/mL to the detection limits of nitrite and nitrate. Quantitation was carried out by the peak area method. The relative standard deviation for the analysis of nitrite and nitrate was less than 3.0%. This method was applied for the simultaneous determination of nitrite and nitrate in dew, rain, snow and lake water samples collected in southeast Massachusetts. High levels of nitrite ( > 0.6 µg/mL) and nitrate ( > 4.7 µg/mL) were observed in dew water droplets. |
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The Biogeochemical Cycling of Nitrogen at Various Spatial and Temporal Scales
1:30 PM-4:40 PM, Monday, August 20, 2007 Boston Park Plaza -- St. James Rm, Oral
Sci-Mix
Division of Geochemistry |