Metabolic phenotypes for MCF-10A cancer cell lines

AEI 2

Mădălina Ciobanu, madalina.ciobanu@vanderbilt.edu and David E. Cliffel, d.cliffel@vanderbilt.edu. Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, TN 37235-1822
Cancer is one the main diseases of interest for researchers. Correlating genetic mutations within the cells with their metabolic phenotype, e.g., glycolysis rate, is important for understanding the disease. We have used multianalyte microphysiometry (MAMP) to measure glycolytic rates for cancer cell lines derived from MCF-10A. We can measure simultaneously glucose uptake, oxygen consumption, lactate production and acidification rate with the MAMP. The more aggressive the cancer cell line is, the higher the lactate production, for example, and consequently the higher the acidification rate. These results indicate upregulated glycolysis for cancer cells, in agreement with the Warburg effect. The results provide vital data to enable mathematical modeling of cancer.