AEI 44 |
| Although most analyses of human and other genomes limit their focus to the linear sequence of nucleotides, this may neglect DNA structural features that are crucial for recognition by a regulatory protein. Recently, our laboratory developed a computational method, Conserved-OH-Radical-Cleavage-Signature (CORCS) analyses that uses Gibbs sampling to find common structural features in functional genomic DNA elements that are not similar in nucleotide sequence. Previously, we used CORCS to show that DNaseI hypersensitive sites, which mark regions of open chromatin, have common DNA structural motifs but no apparent sequence consensus. We have now applied CORCS to a collection of experimentally defined transcription factor binding sites in the human genome that contain little or no enrichment of consensus sequence motifs. We chose this set because, surprisingly, they contain little or no enrichment for consensus sequence motifs. Application of CORCS to other functional annotations, such as enhancers, will provide further insights into how DNA mediates function in the human genome. |
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Academic Employment Initiative
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix
Academic Employment Initiative |