Affinity adsorption of viruses

AEI 28

Caryn L Heldt, clheldt@ncsu.edu1, Patrick V. Gurgel2, Lee-Ann Jaykus, leeann_jaykus@ncsu.edu3, and Ruben G Carbonell, ruben@ncsu.edu1. (1) Department of Chemical and Biomolecular Engineering, North Carolina State University, 911 Partner's Way, Raleigh, NC 27695, (2) ProMetic Life Sciences, 8168 chemin Montview, Mont-Royal, QC H4P 2L7, Canada, (3) Department of Food Science, North Carolina State University, Schaub Food Science Building 339A, Campus Box 7624, Raleigh, NC 27695
The capture of viruses from complex sample matrices is necessary for many different applications, including the removal of viruses from process streams, concentration of viral vectors for gene therapy, and molecular surface detection for a sensor. Small peptides, RNA riboswitches, and other olignionucleotide constructs could be used to create specific recognition surfaces that identify the presence of viruses. For example, small peptide sequences have been identified for the capture of porcine parvovirus (PPV). These peptides have been used in chromatographic columns for removal of PPV from complex mixtures containing human blood plasma. This approach is a promising alternative to virus capture using antibodies because it eliminates the risk of viral contamination that may be found in antibody preparations. Furthermore, peptides are inexpensive to produce and more stable than antibodies. In the future, other constructs could be discovered for the detection of viruses and toxins from different environmental samples.
 

Academic Employment Initiative
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Academic Employment Initiative

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007