CHED 201 |
| Breast cancer is the most deadly disease in women. Although primary human breast cancer-derived epithelial cells (HBCEC) can be maintained in culture for at least two years and still proliferating, data on the properties of these tumor cells that distinguish them from their normal, non-tumorigenic counterparts remain scarce. Thus, FACS-analysis of cell surface markers in HBCEC will be performed to characterize these tumor cells in comparison to normal human mammary epithelial cells (HMEC). A similar analysis in human breast cancer cell lines (e.g. MCF-7, MDA-MB-231) will examine potential differences between the artificially immortalized cell lines and HBCEC cultures. Differences in intracellular pathways that appear will be further investigated using Western blot analysis and 2D-PAGE, and RNAi may be used to study factors involved in these signalling pathways. Fluorimetric assays will examine distinct enzyme activities in the tumor cell populations compared to HMEC depending on culture conditions and exogenous stimulation. |
|
Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Chemical Education |