ANYL 336 |
| Coherent anti-Stokes Raman Scattering (CARS) microscopy is maturing as a powerful imaging tool capable of real-time point-by-point chemical maps of living cells and tissues, without the need for labels or stains. In contrast to single-frequency CARS imaging, broadband CARS imaging is capable of measuring subtle changes in the vibrational fingerprint region. We have previously demonstrated that a single-laser broadband CARS microscope is able to acquire a vibrational spectrum of 3000 wavenumbers in a single exposure. I will present a new approach to broadband CARS generation controlling timing and chirping of pulses. Additionally, I will discuss its potential for greater sensitivity and effective suppression of non-resonant background. |
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Biological Applications of Nonlinear Optics
8:30 AM-12:10 PM, Tuesday, August 21, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |