AGRO 6 |
| Persistent organic pollutants (POPs) including polycyclic aromatic hydrocarbons (PAHs), pyrethroids, and polychlorinated biphenyls (PCBs) present a growing environmental and human health concern. Reliable and cost effective analytical methods for POPs are required for large-scale environmental monitoring studies. Immunoassay and immunoaffinity purification procedures are sensitive antibody-based bioanalytical methods that provide many advantages over conventional instrumental and traditional cleanup methods. Enzyme-linked immunosorbent assay (ELISA) methods have been developed for monitoring PAHs, permethrin and PCBs, in complex sample media. The method precision for PAH ELISA was within ±30% and recovery of benzo[a]pyrene in ranged from 46 to 140% for soils. The ELISA data were highly correlated with data obtained from the GC/MS (r > 0.77). Another magnetic particle ELISA for measuring permethrin in soil and dust giving a positive correlation (r = 0.99) with GC/MS data. Overall method precision was within ±30% and recoveries of mixtures of cis- and trans-permethrin (1:1) ranged from 92 to 116%. ELISA methods for Aroclors (96-microwell plate) and coplanar PCBs (magnetic particle) were evaluated for soil and sediment. A positive correlation (r = 0.90 for Arochlors and 0.66 for coplanar PCBs) was observed between ELISA and GC/MS data. Notice: The United States Environmental Protection Agency (EPA) through its Office of Research and Development funded and collaborated in the research described here under contracts 68-D4-0023 and 68-D-99-01 to Battelle. Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. |
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Immunochemistry
8:40 AM-12:05 PM, Sunday, August 19, 2007 BCEC -- 259A, Oral
Division of Agrochemicals |