Enantioseparation of three chiral compounds by Capillary Electrophoresis used cyclodextrin chiral selector as chiral mobile phase additives

ANYL 131

Rongji Dai1, Lin Yang2, Wei Liu, liuwei821215@yahoo.com.cn2, Yulin Deng3, Weiwei Meng1, and Haihui Wang, whh886_1@yahoo.com.cn2. (1) School of life science and technology, Beijing Institute of Technology, 5 South Zhongguancun Street Beijing,P R China, Beijing, 100081, China, (2) school of life science and technology, Beijing institute of technology, No. 5, Zhongguancun Nandajie, Haidian District, Beijing 100081, P. R. China, beijing, 100081, China, (3) School of Life Science & Technology, Beijing Institute of Technology, China, NO. 5 Zhong Guan Cun NAN Street, 100081 Beijing, China
2-HPA is a new drug to cure the Parkinson's disease. In order to separate 2-HPA we found that separation of 2-HPA by capillary electrophoresis was efficient. Further more the precursor 2-HA and outgrowth 2-HPPA were separated. Dansyl chloride was chosen to derivatize 2-HPA and 2-HA. 2-HPPA was separated directly. A suitable derive and chiral analysis conditions in capillary electrophoresis was studied. The experiment proved that the speed of isomer moving toward to cathode decelerated under the low pH condition, so the coalescent time between β-cyclodextrin and isomer were prolonged. On the contrary, higher pH was disadvantageous in separation .We confirmed that the best separate pH condition was 4.34. The effect of chiral selector was important also. we choose the β-cyclodextrin concentration 10mmol/l. Under the optimal condition 2-HPA-Dns was separated by based-line, 2-HA-Dns and 2-HPPA were separated well.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007