Seperation and determination of glycyrrhizic acid and liquiritin in licorice using SPE-RP-HPLC

ANYL 245

Yuhong Yu, yyh100218@sohu.com1, Jianjun Cai1, Rongji Dai, dairongji@bit.edu.cn2, Yulin Deng3, Kun Yang2, and Weiwei Meng1. (1) School of life science and technology, Beijing Institute of Technology, 5 South Zhongguancun Street Beijing,P R China, Beijing, 100081, China, (2) School of life science and technology, Beijing Institute Of Technology, 5 South Zhongguancun Street Beijing,P R China, Beijing, 100081, China, (3) School of Life Science & Technology, Beijing Institute of Technology, China, NO. 5 Zhong Guan Cun NAN Street, 100081 Beijing, China
Glycyrrhizic acid (GA) and Liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our study , Solid-phase extraction (SPE) were compared with Liquid¨Cliquid extraction (LLE), sulphuric acid precipitation and Polyamide resin column separation. Solid-phase extraction method was verified to separate GA and Liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control of drug. Moreover, we built a new method to determine GA and Liquiritin simultaneously rely on Reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for Liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3% and 99.4%£»RSD were 1.01% and 0.92%.
 

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7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

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Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007