Analysis of commercially available antibodies to hepatitis B surface antigen

CHED 200

Colleen McCage1, Oanh Ngo1, Christine E. MacTaylor, christine.mactaylor@salemstate.edu1, and Alynne MacLean2. (1) Department of Chemistry and Physics, Salem State College, 352 Lafayette St, Salem, MA 01970, (2) Science With a Mission, 602 Massapoag Ave, Sharon, MA
Gel electrophoresis using 4-20% tris-glycine polyacrylamide gel and nitrocellulose transfer paper was used to test commercially available hepatitis B surface antigen antibodies [anti-HBsAg]. Antigens of various subtypes were run on the gels along with molecular wieght markers. After speparating on the gels, the antigens were transferred to the nitrocellulose and probed with four different antibodies for HBsAg. Three rabbit polyclonal antibodies and one mouse monoclonal antibody were tested in order to determine which subtypes of HBsAg the antibodies would bind.
 

Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Chemical Education

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007