CHED 90 |
| This presentation will describe a new method for analyzing ascorbic acid using bicinchoninic acid and cupric ions. The cupric ion oxidizes ascorbic acid while being reduced to cuprous ion. One equivalent of the cuprous ion combines with two equivalents of the ionized bicinchoninic acid, giving a very deep purplish color. A standard curve is used to determine the concentration of the ascorbic acid. Using either a microplate reader or a visible spectrometer, the method is quick and easy, but not specific for only ascorbic acid. There are several substances which could interfere with the analysis and so there are limitations as to which ascorbic acid-containing samples can be analyzed. Comparisons will be made with other methods along with how to avoid interference. |
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General Posters
7:30 PM-9:30 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Chemical Education |