BIOL 121 |
| The activation of gene expression involves a wide array of protein-protein interactions between transcriptional activators and co-activators leading to the recruitment of RNA polymerase II to the gene promoter. Transcriptional activators interact with a variety of proteins in vitro; however, only a subset of these interactions is physiologically significant. We aim to resolve these interactions using site-specific chemical crosslinking in conjunction with mass spectrometry. Specifically, we aim to not only identify the relevant activator targets but more importantly, determine the activator binding sites on these proteins. We have incorporated a photo-reactive nonnatural amino acid, p-benzoylphenylalanine, into the activation domains of VP2, Gcn4, and Gal4 and crosslinked these activators with yeast transcriptional machinery proteins. Identification of the direct activator binding sites on relevant targets will provide further insight into the mechanism of transcriptional activation as well as reveal pertinent screening targets for the design of therapeutically useful activator artificial transcription factors.
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Poster Session
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |
