Antisense cassette for gene expression imaging

BIOL 168

Huafeng Fang, hfang@artsci.wustl.edu, Bereket Y. Oquare, byoquare@wustl.edu, Yanming Zhang, yzhangb@artsci.wustl.edu, and John-Stephen Taylor, taylor@wustl.edu. Department of Chemistry, Washington University in Saint Louis, One Brookings Drive, cb # 1134, St. Louis, MO 63130
In the past decade, the ability to fuse proteins with fluorescent proteins and peptide tags has enabled a wide range of studies of specific proteins in living cells with absolute specificity. By contrast, there are few reports of methods for tagging mRNAs for in vivo imaging. Herein, we report the design and synthesis of short repeated RNA sequences (antisense cassettes) for tagging mRNA transcripts and show that can then be imaged in living cells by a 2-OMe RNA molecular beacon probe. (This material is based upon work supported by the National Heart Lung and Blood Institute of the National Institutes of Health as a Program of Excellence in Nanotechnology (HL080729)).
 

Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biological Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007