Development of label-free arrayed SPR sensors for food safety monitoring

ANYL 20

Allen D. Taylor, adtaylor@u.washington.edu1, Jon Ladd1, Stacey Etheridge2, Jiri Homola3, and Shaoyi Jiang, sjiang@u.washington.edu1. (1) Department of Chemical Engineering, University of Washington, Seattle, WA 98195, (2) 2Center for Food Safety and Applied Nutrition, US Food and Drug, College Park, MD 20740, (3) Institute of Radio Engineering and Electronics, Academy of Sciences, 18251 Prague, Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
There is an urgent need for fast, sensitive, and reliable detection methods for food contaminants from both inadvertent food contamination and deliberate terrorist attacks. Surface plasmon resonance (SPR) biosensor is well suited for this purpose since it provides fast, quantitative, label-free, and real-time detection. This talk will focus on recent developments in (a) SPR instrumentation, particularly arrayed SPR imaging sensor systems, (b) protein arrays, particularly those created from DNA arrays and those based on superlow fouling zwitterionic-based materials, and (c) detection assays for the simultaneous and quantitative detection of large-sized analytes (e.g., Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Campylobacter jejuni), medium-sized analytes (e.g., Staphylococcal Enterotoxin B, botulinal toxins A, B, E, and F), and small-molecular-weight compounds (e.g., domoic acid, saxitoxins, and tetrodotoxin) in complex media (e.g., apple juice, milk, urine,pufferfish extract, and shellfish extract).
 

Analysis of Chemical Contaminants in Foods and Food Contact Substances
8:30 AM-12:00 PM, Sunday, August 19, 2007 BCEC -- 104A, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007