Analysis of mitochondrial distribution within muscle fibers as an age dependent factor using direct histological sampling interfaced with capillary electrophoresis with laser-induced fluorescence detector

CHED 173

Stella P. Hartono, sphartono@csupomona.edu and Hossein Ahmadzadeh. Department of Chemistry, California State Polytechnic University, Pomona, 3801 West Temple Avenue, Pomona, CA 91768
Aging is associated with a progressive decline of muscle mass, strength and quality. Mitochondrial mutation and degeneration has been proposed as the underlying cause of this condition due to high generation of reactive oxygen species and loss of ATP production. It is not clear whether Type I or Type II fibers initiate the mitochondrial dysfunction associated with aging. This project proposes to determine if the earliest sign of mitochondrial dysfunction occurs within Type I or Type II muscle fibers by analyzing mitochondrial distribution along single muscle fibers of rats of varying age using direct sampling of tissue cross sections followed by capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detector which allow a highly sensitive analysis of individual mitochondria within a single muscle fiber.
 

Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Chemical Education

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007