CHED 206 |
| Malignant melanoma is responsible for 75%-90% of all skin cancer related deaths. The development of antibodies for the detection of specific melanoma biomarkers would greatly increase the ease and speed of detection and could lead to methods for routine monitoring of patients following surgical treatment. 5-S-cysteinylydopa (5-SCD) has been shown to be a good diagnostic marker of malignant melanoma; however its oxidative instability makes it difficult to test in plasma or urine. An immunoassay for 5-SCD would provide an effective and economical method for the detection of malignant melanoma in skin cancer patients. Previous attempts to identify an antibody for 5-SCD have demonstrated limited selectivity. We plan to identify antibodies with high affinity and specificity using traditional immunology techniques. We are synthesizing a 5-SCD analog containing a biotin moiety for a spectroscopic immunoassay to validate binding. We plan to use an electrochemical immunoassay to determine 5-SCD concentrations in samples. |
|
Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Chemical Education |