Supported planar lipid bilayers on step-and-terrace TiO2 surfaces

COLL 207

Ryugo Tero, tero@ims.ac.jp1, Toru Ujihara2, and Tsuneo Urisu, urisu@ims.ac.jp1. (1) Department of Biochemistry and Coordination Chemistry, Institute for Molecular Science, Myodaiji, Okazaki, aichi, 444-8585, Japan, (2) Department of Crystalline Materials Science, Graduate School of Engineering, Nagoya University, Furocho, Chikusaku, Nagoya, 464-8603, Japan
We studied the influence of substrate surface properties on supported planar bilayer (SPB) using atomic force microscope and fluorescence microscope. Effects of surface hydrophilicity and atomic structures on the SPB formation process, morphology, and phase-separation were investigated on single-step-and-terrace rutile-TiO2 low index surfaces. Step-and-terrace surfaces of rutile-TiO2(100), (110) and (001) were formed by etching in 10% HF aq. and annealing at O2 flow (1.0 L/min) at 700-850°C. Flat and continuous SPB was formed on both TiO2(100) and (001) surfaces by the vesicle fusion method. The dipalmitoleoylphosphatidylcholine (DPoPC)-SPB on the single- and double-step TiO2(100) had a ratchet-like structure following the step-and-terrace structure of the substrate, but that on the half-step TiO2(001) did not have the morphology reflecting the substrate structure. The effects of substrate structures on the domain shapes in the binary bilayer of DPoPC and dipalmitoylphosphatidylcholine (DPPC) will be discussed.
 

Fundamental Research in Colloid and Surface Chemistry
6:00 PM-8:00 PM, Monday, August 20, 2007 BCEC -- East Registration, Poster

Division of Colloid & Surface Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007