ANYL 442 |
| New miniature, multifunctional, and sensitive sensor platforms are required for applications ranging from the characterization of photon-driven proton pumping in biomimetic photosynthetic devices, to the sensing of drug/membrane protein interactions and chemical warfare agents. Our vision for such sensor systems is to combine optical and electrochemical modes of signal transduction to probe the interface between the sensor architecture and the surrounding environment. Our group has recently shown, using electroactive planar waveguides, the power of combining spectroelectrochemical characterization of redox events, for surface-adsorbed redox active molecules, and for redox molecules interfaced with planar supported lipid bilayers. Our most recent efforts have focused on the development of fiber-optic chip (FOC) spectrometers as sensor platforms. The FOC combines the sensitivity of an attenuated total reflectance (ATR) system with the ease of use of fiber-optic based CCD spectrometers. We use a side-polished optical fiber, in a V-groove glass mount, to create a planar platform, which allows access to the evanescent field escaping from the fiber core. The exposed evanescent field is used to probe molecules or molecular assemblies supported by the platform over a broad wavelength range, and it has an interaction area of 0.05 cm2. This presentation will focus on our efforts to optimize optical detection of surface bound proteins absorbing in the UV, visible back-coupled fluorescence detection of a binding event, and spectroelectrochemical modulation. |
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Analytical Approaches
9:00 AM-12:00 PM, Thursday, August 23, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |