Toward multiplexed magnetic resonance imaging with functionalized xenon-129 biosensors

INOR 786

P. Aru Hill, hillp@sas.upenn.edu1, Qian Wei1, Garry Seward1, Nicholas Kuzma2, One-Sun Lee, one@sas.upenn.edu1, Jeffery G Saven1, and Ivan J. Dmochowski, ivandmo@sas.upenn.edu1. (1) Department of Chemistry, University of Pennsylvania, 231 S. 34th St., Philadelphia, PA 19104, (2) Department of Biomedical Engineering, University of Rochester, Rochester, NY
Xenon-129 biosensors offer an attractive alternative to conventional MRI contrast agents due to the chemical shift sensitivity and large nuclear magnetic resonance signal of hyperpolarized 129Xe. To help realize the potential of this technology, two complementary analytical techniques: fluorescence spectroscopy and isothermal titration calorimetry (ITC) are used to determine the xenon binding association constant of a water-soluble triacidic-cryptophane-A (I). These techniques both give a binding affinity of 17,000 at 293 K, indicating that I has the highest known affinity for xenon to date. These studies have guided the design of new xenon-binding cages. Also, conjugation of receptor-specific and cell-penetrating peptides to our xenon-binding cages has allowed for cell localization and toxicology studies. Finally, these studies further the understanding of the nonbonding interactions of this main group element.
 

Main Group Chemistry
9:00 AM-12:20 PM, Wednesday, August 22, 2007 BCEC -- 205C, Oral

Division of Inorganic Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007