COLL 156 |
| Supported lipid membranes have attracted huge interest for their properties as models of cell membranes and for potential applications in biotechnology. The formation and stability of lipid bilayers can depend on many factors such as the type of substrate, pH, ionic strength and type of ions in the solution etc. We have investigated the effects of surface silanol density and surface curvature on the formation of DPPC bilayer membranes on SiO2 nanoparticles with a size range from 5-100nm. Surface silanol density was changed by a combination of heat and acid treatment and monitored using FTIR spectroscopy. Sub-bilayer DPPC adsorption, measured using thermogravimetric analysis, was observed at low silanol density, but at high silanol densities, bilayer coverage was obtained for all bead diameters, both for incubation of vesicles/beads above and below the DPPC phase transition temperature, Tm. Differential scanning calorimetry showed a broadening of Tm for the adsorbed bilayers. Raman spectroscopy of the supported bilayers showed that the dissorder of the alkane chains increased with deacreasing diamether of the nanoparticles. |
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Fundamental Research in Colloid and Surface Chemistry
6:00 PM-8:00 PM, Monday, August 20, 2007 BCEC -- East Registration, Poster
Division of Colloid & Surface Chemistry |