BIOL 93 |
| One of the major research areas in the field of cell-based biosensors is the development of functional cell-based sensor networks and their integration with silicon-based platforms. Using surface chemistry, we have developed a technique to modify the surface of commercially available microelectrode arrays using self-assembled monolayers (SAM), and then pattern them using a photolithography based method in order to guide neuron attachment and development. Dissociated embryonic hippocampal cells in a serum-free medium were used on these patterns to create neuronal networks with directed synaptic connectivity. The functionality of these networks was further studied using long term recording of the electrical activity of these cells. We have also developed a technique to study myocardial tissue function by manipulating surface chemistry to pattern and guide the growth of spontaneously beating monolayers of neonatal rat cardiomyocytes on glass. These hybrid systems can be used for studying basic neuronal networks and cardiac physiology. More importantly they could find applications in toxin detection, pharmacological testing and to study disease models.
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |
