TOXI 39 |
| A strong association between chronic inflammation and cancer risk has been shown in many human and animal studies, but the chemistry underlying this association is largely undefined in cells and tissues. One model posits generation of reactive oxygen, nitrogen and halogen species by macrophages and neutrophils migrating to sites of inflammation, with these species damaging DNA, RNA, proteins and lipids in neighboring host cells. We recently demonstrated that etheno adducts in DNA were the only DNA lesions showing significant increases in tissues from the SJL mouse model of inflammation. We have now extended these biomarker studies to RNA. Using a novel and sensitive LC/MS-MS method, we quantified the RNA damage products representing the broad classes of inflammation chemistry: three deamination products, one halogenated base, and three unsubstituted etheno adducts. The results highlight the utility of RNA adducts as biomarkers of inflammation. |
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Poster Session and Awards
6:00 PM-10:00 PM, Tuesday, August 21, 2007 BCEC -- 204 A/B, Poster
Sci-Mix
Division of Chemical Toxicology |