CHED 327 |
| DNA hybridization studies are integral tools for the diagnosis of genetic diseases and the detection of biological pathogens. Providing versatile methods for the site-specific incorporation of spectroscopic and redox-active reporter groups in DNA is an important component in the development of effective diagnostic methods. In particular, labeling strategies that utilize chemistries compatible with the reagents and conditions employed during solid-phase oligonucleotide synthesis are very desirable. We are developing an on-column derivatization method for the synthesis of labeled DNA. In this method, a nucleoside containing a protected thiol moiety is incorporated into a synthetic DNA. The thiol group can be deprotected and functionalized while still on the solid support, or cleaved from the column prior to labeling. The synthesis and characterization of a uridine nucleobase containing a protected thiol will be presented. Initial strategies for the synthesis and labeling of oligodeoxynucleotides will also be discussed. |
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Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Chemical Education |