Photopolymerized silica sol-gel affinity monoliths for on-chip separations

ANYL 213

Miriam H. Levy, levym2@rpi.edu, Shubhodeep Goswami, goswas@rpi.edu, Joel Plawsky, plawsky@rpi.edu, and Steven M. Cramer, crames@rpi.edu. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180
Affinity monoliths using 3-methacryloxyproplytrimethoxysilane as a monomer precursor were fabricated using UV irradiation at 365 nm. The stationary phase was further derivatized with Cibracon Blue F3GA to enable affinity capture of FITC labeled lysozyme and albumin. The efficacies of different immobilization techniques were examined in batch to determine systems with the highest capacity and the least non-specific adsorption. Characterization of the stationary phase was performed using optical microscopy, scanning electron microscopy and nitrogen BET. Electroosmotic flow was employed for fluid flow and samples were injected by pulsed injection in these chip systems. Finally, proof of concept on-chip affinity capture was demonstrated using laser induced fluorescence detection.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007