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| Identification of the protein-protein interactions within multi-protein complexes is crucial to understand their mechanisms. Unique affinity separation techniques coupled with cross-linking will directly minimize background contamination and improve MS signal/noise and sensitivity in mass spectrometry. In this work, functional cross-linkers were synthesized by using 5-hydroxy 2-nitrobenzyl alcohol and 2-mercaptosuccinic acid as the starting materials. The linker will be react with model proteins such as Hemoglobin and tubulin dimers and then produce protein-protein cross-linkings in the complexes. The photo cleavable cross-linkers were designed to anchor/detach the cross-linked peptides by modified beads. In the future, the special protein-protein interactions in the interested parasites, T. gondii and C. parvum, will be investigated. (Supported by NIH/NIAID contract HHSN266200400054C)
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General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Analytical Chemistry |