Nature of covalent adducts formed on ds-DNA by dirhodium(II,II) carboxylate compounds

INOR 150

Shari U. Dunham, sharidunham@moravian.edu and Stephen U. Dunham, stephendunham@moravian.edu. Department of Chemistry, Moravian College, 1200 Main Street, Bethlehem, PA 18018
Dirhodium(II,II) carboxylate compounds covalently bind to duplex DNA. Control of DNA-binding kinetics and intra/inter-strand DNA adduct profile is dependent upon the number and nature of the bidentate carboxylate ligands. We have further characterized the nature of these covalent Rh-DNA adducts by reaction of double-stranded salmon testes DNA with a series of dirhodium (II,II) compounds followed by digestion with DNaseI, P1nuclease, and alkaline phosphatase enzymes. Nature of DNA adducts formed by these dirhodium compounds will be discussed as characterized by rhodium AAS, HPLC and mass spectrometry.