Colorimetric analysis of nitric oxide donors for the determination of nitric oxide release profiles

ANYL 86

Chanda L. Elam, celam@uakron.edu, Department of Chemistry, University of Akron, 302 Buchtel Common, Akron, OH 44325-3601
The kinetic profiles of diazeniumdiolate nitric oxide (NO) donors are often determined through the use of a spectrophotometer or nitric oxide analyzer (NOA). However, in the case of the water insoluble lipophilic diazeniumdiolates, the kinetic profiles cannot be measured directly under physiological conditions. Another problem arises when dealing with both lipophilic and polymeric donors, which release NO over longer periods of time; the NOA frequently underestimates the total NO release for such compounds. In this work, a spectrophotometric method has been devised that gives a continuous measurement of NO release from insoluble donors. The sample uses phase intermediate contact with the colorimetric solution to evolve the nitric oxide, which is then analyzed over time using the UV/VIS spectrometer. This method has established a viable means to analyze samples that were once unable to be accurately characterized.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007