Simultaneous saccharification and cofermentation of paper sludge to ethanol by recombinant xylose-fermenting microbes


Jiayi Zhang, and Lee R. Lynd, Thayer School of Engineering, Dartmouth College, 8000 Cummings Hall, Hanover, NH 03755
As a waste produced from the paper making that does not require pretreatment in many cases, paper sludge is an attractive raw material for the emergent cellulosic ethanol industry as well as a model substrate for investigation of simultaneous saccharification and co-fermentation (SSCF) of glucose and xylose. Experimental work is described using two promising recombinant, microbes engineered to be able to utilize xylose: Z .mobilis 8b and S. cerevisiae RWB222. SSCF of paper sludge at concentrations sufficient to produce > 40 g/L ethanol is evaluated at about 30oC and 37oC, and performance is compared with respect to hydrolysis yields, sugar utilization, and cell viability. Compared to strains able to utilize glucose only, SSCF allows ethanol yields from paper sludge to be increased by about 20%.

Biofuels: Lignocellulosic Biofuels
1:15 PM-5:10 PM, Monday, August 20, 2007 Boston Park Plaza -- Arlington Rm, Oral

Division of Fuel Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007