ANYL 211 |
| In our previous studies, we have developed a glucose indictor (GIP)and used it for continuous monitoring of glucose concentration. In this study, we present a new type of GIPs that can tolerate to pH changes between pH 7.4 to 5.5. As the intracellular pH varies within living cells, The pH insensitive GIPs will be useful for the determination of intracellular glucose concentration through FRET fluorescence imaging measurement of GIPs which genes are integrated to the chromosome of the target cells through retrovirus infection. Our experiment results demonstrated that the engineered GIP is less affected by pH, making it ideal for use in determining the intracellular glucose within living cells using fluorescence lifetime imaging microscopy measurement.
|
|
General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Sci-Mix
Division of Analytical Chemistry |