Award Address (Spectrochemical Analysis, sponsored by the Division of Analytical Chemistry). Ultrasensitive biosensing with surface plasmons, surface enzyme chemistry, nanoparticles and diffraction gratings

ANYL 313

Robert M. Corn,, Hye Jin Lee,, and Alastair Wark, Department of Chemistry, University of California-Irvine, Irvine, CA 92697-2025
Surface bioaffinity biosensors have become invaluable biotechnological tools for the rapid, multiplexed detection of biomolecules. In the last decade, a number of surface-sensitive spectroscopic techniques based on changes in the local optical index of refraction upon adsorption have emerged as attractive alternatives to traditional fluorescence-based detection methods for surface bioaffinity biosensing. For example, we have successfully applied surface plasmon resonance imaging (SPRI) to measure the bioaffinity adsorption of DNA, RNA, antibodies, proteins and biomarkers. In this talk we will describe our recent efforts to create the next generation of ultrasensitive biosensors which use a combination of surface enzyme chemistry and nanoparticle surface incorporation. For example, DNA microarrays, nanoparticles and poly(A) RNA polymerase can be used for microRNA profiling with SPRI at femtomolar concentrations. In addition, we will also describe a new optical technique, nanoparticle-enhanced diffraction gratings (NEDG), that can be used to create novel ultrasensitive biosensors.