CHED 209 |
| The Chemical Shift Index method (CSI) uses random coil chemical shift references as an efficient and economical method of analyzing secondary structures in proteins. However there are many environmental (pH, chaotropic agent) and structural (neighboring amino acids) factors, which can affect the α-proton chemical shifts, and therefore the accuracy of CSI. Correction factors can account for the changes in the α-proton chemical shifts caused by these different variables. Therefore, the present study will investigate the affects of pH, urea and neighboring acidic and basic amino acids on the α-proton random coil chemical shift values of alanine to determine a set of correction factors. All experiments are performed using pentapeptides with the structure H2N-Gly-Xaa-Ala-Yaa-Gly-CO2H (Figure 1). 1H NMR and 2D COSY, TOCSY and NOESY experiments are used to determine α-proton chemical shifts, verify amino acid sequence and confirm the random coil state of the peptides.
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Undergraduate Research Poster Session
2:30 PM-4:30 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Chemical Education |
